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Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, <t>microRNA;</t> pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.
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Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, <t>microRNA;</t> pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.
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Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, <t>microRNA;</t> pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.
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Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, <t>microRNA;</t> pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.
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Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, <t>microRNA;</t> pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.
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Image Search Results


Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, microRNA; pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.

Journal: bioRxiv

Article Title: Differentially deregulated microRNAs contribute to ultraviolet radiation-induced photocarcinogenesis through immunomodulation: An-analysis of microRNAs expression profiling

doi: 10.1101/2023.02.24.529976

Figure Lengend Snippet: Biogenesis of miRNAs. (A) In the nucleus, ribonuclease enzyme II transcribes the pri-miRNA transcript. Drosha and its co factor Pasha form the microprocessor complex that cleaves pri-miRNA transcript into pre-miRNA which share a stem loop structure. These pre-miRNAs are transported by exportin-5 into cytoplasm from nucleus. (B) In the cytoplasm, Dicer unwind the miRNA duplex into mature miRNA and complementary strand (miRNA*, which rapidly degraded), and loaded mature miRNA into RNA-induced silencing complex (RISC). (C) Depending on the degree of complementarity between the target mRNA and the miRNA, the binding ether stops the translation by cleaving the target mRNA or suppress translation by binding to impeding ribosomal reading of the mRNA (incomplete complementarity). miRNA, microRNA; pri-miRNA, primary microRNA; pre-miRNA, precursor -microRNA; RISC, RNA-induced silencing complex.

Article Snippet: The miRCURY LNA ™ microRNA array slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA), and the image analysis was carried out using the ImaGene ® 9 (miRCURY LNA ™ microRNA Array Analysis Software, Exiqon, Denmark).

Techniques: Binding Assay